ABSTRACT
A method for rapid determination of γ-hydroxybutyric acid (GHB) in beverages (water, sodas, beer) and urine was established by direct analysis real-time mass spectrometry (DART-MS). Samples were analyzed directly after dilution with mixture of methanol and water(1:1,V/V). Instrument parameter settings were optimized to obtain the sensitive and accurate determination of GHB. At the sample introduction speed of 0.5 mm/s, high intensity of[M-H]- ions for GHB were observed in the negative ion and selection ion monitoring mode by utilization of high purity helium gas at 350℃. For different samples of water,sodas,beer and urine,the limits of detection (LODs) (S/N=3) were in the range of 1-2 μg/mL, while the limits of quantification (LOQs) (S/N=10) were in the range of 3-5 μg/mL. The linear correlation coefficients of the standard curves with different sample matrixes were between 0.9899 and 0.9980. The recoveries were in the range of 80.8%-115.2% with the relative standard deviations of 1.9%-12.8%. With its rapid analysis and simple pretreatment steps,the method is expected to have a strong advantage in the rapid screening analysis of large quantities of beverage and urine.
ABSTRACT
Recently, much attention has been given to develop simple analytical methods for screening wide range of date-rape drugs. Drug abuse that changes human behavior and guides to crimes has become a serious problem throughout the world. The determination of various classes of abused drugs is an important in many fields of analytical toxicology, such as forensic analysis and antidoping analysis. The detection method must provide more accurate and reliable results from the test specimens. In this regard, attempt is made in this review to summarize the recent applications of these methods in keeping track with complexity of the sample matrices. The following topics are discussed under the headings of different sampling approaches for screening the drugs related to drug facilitated sexual assault and more commonly utilized analytical methods such as liquid chromatography, gas chromatography and capillary electrophoresis separation techniques coupled with mass spectrometry detector. Therefore, relentless efforts should continue to be deployed toward research and development in low cost, fast and efficient ways of detecting illicit drug from all kind of specimens for reliable and more accurate results.